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Figure 3–25. Electron micrograph of a cell with five nuclei in Scenedesmus acutus (Nicolov et al., 1986).
There was performed a cytophotometric measuring of the amount of
DNA by recording the luminescent intensity of DNA-bounded bisbenzimide H-
33258. Fourteen trinuclear cells (3n) were subjected to photometry
individually. After statistical proceeding of the results the cells are distributed
in three groups (I, II, III) by ascending value. The data of this study expressed
in relative units are given diagrammatically (Fig. 3–26). As seen from the
diagram the nuclei of group III demonstrate higher, almost double DNA
quantity (18.22) than that of group I (9.58). The nuclei of group II are much
more closer to group I (11.22:9.58), than to those of group III (11.22:18.22).
These results give grounds to accept that in the presence of three nuclei in
one cell, one of them is in a more advanced phase of development than the
other two, which differ less in DNA-amount and most probably are in earlier
phases of preparation for division.